Epithelial-associated Macrophages, Gut (MT-0224-HM17)

Yes. Our isolation strategy is optimized to preserve the tolerogenic and barrier-supportive phenotype characteristic of epithelial-associated macrophages. We evaluate basal cytokine secretion, expression of key surface markers, and responses to microbiota-related stimuli. Under recommended conditions, the cells exhibit low spontaneous inflammation yet remain responsive to environmental cues.

We use an optimized cryopreservation medium and controlled-rate freezing protocol to minimize phenotype drift and cell damage. Before release, a representative vial is thawed, and we assess viability, morphology, and key functional markers. The product is shipped on dry ice with clear handling instructions. When customers follow our thawing and culture guidelines, the cells typically recover quickly and maintain their epithelial-associated features.

Yes. Our scientists can advise on model design, including co-culture formats, media formulations, and suitable readouts for mucosal immunity and barrier function. While we do not provide fully customized protocols for every scenario, we can share best practices and discuss potential pitfalls.