Macrophages play a crucial role in the immune system, functioning as key players in the body's defense against pathogens and tissue repair processes. Different types of macrophage models can provide a one-stop solution for a wide range of studies, including immune response, drug development, and disease mechanisms.

Macrophage Model: Contributing to Our Understanding of Immune Responses and Disease Pathogenesis

• In vitro macrophages exhibit phenotypic plasticity, meaning they can adopt different activation states (M1, M2, etc.) in response to microenvironmental cues.
• In vitro macrophage models enable the investigation of various macrophage functions, including phagocytosis (uptake of particles or pathogens), cytokine secretion (such as TNF-α, IL-6), antigen presentation, and immune modulation.
• In co-culture or conditioned media experiments, in vitro macrophage models can be used to study interactions between macrophages and other immune cells, such as T cells, B cells, dendritic cells, or stromal cells.
• Infection models using pathogens (bacteria, viruses, parasites) allow researchers to study host-pathogen interactions and immune responses. This helps in understanding mechanisms of pathogen recognition, phagocytosis, intracellular killing, and immune evasion strategies.

Fig.1 M1/M2 model of macrophage activation.^1,2

Macrophage Model Development Services at Creative Biolabs

Our macrophage model development service is offered exclusively to scientific and drug development teams to establish and optimize macrophage-related in vitro or in vivo models to facilitate the understanding of biological processes such as immune response, inflammation, infection, and tumor microenvironment.

We offer, but are not limited to, the following macrophage models:

THP-1-based Macrophage Model

Creative Biolabs creates THP-1 macrophage models by differentiating THP-1 cells into macrophage-like cells. We offer phagocytosis assays to assess the phagocytic capacity of differentiated THP-1 macrophages, cytokine production assays, cell-pathogen interactions, drug screening, and immunomodulation to assess cell viability, cytokine production, or other functions.

RAW 264.7-based Macrophage Model

RAW 264.7 is a widely used murine macrophage cell line that originated from tumors induced in BALB/C mice by the Abelson leukemia virus. Creative Biolabs specializes in the development of RAW 264.7-based macrophage models for research purposes, and our scientists and technology platform with expertise in macrophage cell culture and model development.

U937-based Macrophage Model

U937 cells are a human leukemic monocyte lymphoma cell line. These cells exhibit properties of monocytes and can be differentiated into macrophage-like cells. Creative Biolabs develops U937-based macrophage models to provide quality-controlled cell models and characterization services for your biomedical research.

HL-60-based Macrophage Model

HL-60 cells are a type of human promyelocytic leukemia cell line commonly used in biomedical research, particularly in studies related to hematology, immunology, and cancer. Creative Biolabs provides services for the development of HL-60-based macrophage models, including cell culture, cell differentiation, immunological assays, and data analysis.

KG-1-based Macrophage Model

KG-1 cells are a human myeloblastic leukemia cell line derived from the peripheral blood. KG-1 cells have been particularly useful in investigations related to hematopoiesis, leukemia pathogenesis, and drug screening studies. Creative Biolabs develops a macrophage model based on KG-1 cells, which consists of culturing and inducing differentiation into macrophage-like cells.

HEL-based Macrophage Model

HEL cells are primarily used to study erythrocyte differentiation and erythroleukemia. Developing macrophage models from HEL cells is unconventional. However, Creative Biolabs provides services for the development of HEL-based macrophage models to explore the potential of HEL cells for macrophage differentiation.

In Vitro Coculture Model

Creative Biolabs offers advanced in vitro co-culture models that serve as valuable tools for investigating disease mechanisms and evaluating the effectiveness and safety of therapeutic interventions. Our services are compatible with multiple cell types such as T cells, endothelial cells, fibroblasts, and many others. Both primary cells and cell lines are applied to develop targeted coculture models.

We customize models according to customer needs and provide efficient experimental services using the most advanced cell culture, gene editing, and molecular biology tools. In addition to this, we provide macrophage-related services.

• Cell Culture and Differentiation
• Cell Phenotype Identification and Characterization
• Genetic Engineering
• Disease Modeling
• Drug Screening Assay

You can choose the service you need based on your specific macrophage project, or directly click on the keyword to learn more!

Related Products

Our macrophage model development services are designed to provide high-quality and highly customized solutions to meet the diverse needs of our clients in basic and preclinical research. Next, we are pleased to introduce you to our related cell products. These products not only complement our macrophage modeling services, but also provide more comprehensive support for your research.

Service Features

Macrophages from Multiple Sources

We are able to provide macrophage models from different species (e.g., human, mouse, etc.) as well as specific models incorporating various pathological states (e.g., inflammation, tumor microenvironment, etc.) to meet diverse research needs.

Customized Model Development

According to the specific research needs of our clients, we are able to provide customized culture models for different types of macrophages, including primary macrophages, cell lines and transgenic models to meet various experimental requirements.

High-throughput Screening Capability

Utilizing advanced cell culture technology and automated equipment, we are able to perform high-throughput screening to quickly screen out good macrophage models and improve research efficiency.

Multiple Functional Analysis

We provide comprehensive functional analysis services, including macrophage phagocytosis, cytokine secretion, oxidative stress status and other multiple biological property tests, to help customers deeply understand the role of macrophages in disease models.

Workflow of Macrophage Model Development Services

Scientific Resources

Learn More Macrophage Biology

Download M1 vs. M2 Macrophages

Learn More Role and Mechanisms of the CD146+ Macrophage Subpopulation

Learn More Macrophage Culture

Q & A

Q: What is the specific process of macrophage model development service?

A: Our macrophage model development service usually includes several key steps: First, we will communicate with the client to clarify the research objectives and model type. Next, we will conduct literature research and data analysis to ensure the design is reasonable. We then proceed to the experimental stage, where we culture, process and analyze the cells, and finally provide detailed experimental reports and data interpretation to ensure that the client can apply the results to subsequent studies.

Q: What are the advantages of your macrophage model over other models on the market?

A: Our macrophage model has several advantages, including higher physiological relevance and reproducibility. The cell lines we use are carefully selected to better mimic the in vivo environment. In addition, our team has the expertise to provide tailored solutions and insights into experimental design and data analysis.

Q: Can the macrophage models you provide be used in conjunction with other experimental techniques?

A: Our macrophage models have good compatibility and can be used in conjunction with a variety of experimental techniques, including gene editing, drug screening and immunological studies. Our team will propose the best experimental design according to the needs of our clients to fully utilize the synergistic effects of these techniques to study specific biological problems.

Q: Does the development of a customized model take longer than a standard model?

A: Typically, the development process for customized macrophage models may be slightly longer as more communication and design sessions are required. However, we will try to optimize the workflow to ensure that the development time is as short as possible while meeting the customer's needs. The exact timeline will be determined in consultation with the client during the project kick-off meeting.