Morphological Difference Analysis Among Macrophages

As an indispensable part of the host defense system, macrophages exhibit diverse phenotypes and perform intricate roles in both innate and adaptive immune responses. They are presently being evaluated as potential recipients of cell therapy. A throughout comprehension of macrophages is important to promote their application of drug discovery and immunotherapies development. To help global customers in morphological difference analysis of macrophages, Creative Biolabs succeeds in providing a morphological difference analysis service by Gimesa-Wright staining and lysosome staining.

Fig.1 The development process of macrophages.¹

Our Morphological Difference Analysis Service by Gimesa-Wright Staining and Lysosome Staining

The morphological difference analysis service by Gimesa-Wright staining and lysosome staining from Creative Biolabs is a staining-based experiment service. By observing staining and fluorescence density, we are able to analyze relevant morphological differences such as size differences, morphological differences, and lysosomal content differences among diverse macrophages. Furthermore, we provide a well-established macrophage isolation and culture service to enable our macrophage morphological difference analysis service. At Creative Biolabs, we are confident in presenting high-quality macrophage morphological analysis results to support global customers' diverse projects.

Fig.2 Workflow of our morphological difference analysis service by Gimesa-Wright staining and lysosome staining.

Our morphological difference analysis service by Gimesa-Wright staining and lysosome staining is suitable for both primary cells and cell lines. Simultaneously, we provide multiple types of macrophage cell lines to strengthen our morphological difference analysis service:

Fig.3 Macrophage cell lines.

Advantages of Our Morphological Difference Analysis Service by Gimesa-Wright Staining and Lysosome Staining

• Improved Gimesa-Wright dye featuring high sensitivity, rapidness, and stability.
• Improved Gimesa-Wright dye designed to generate a more pronounced staining effect for nuclei and basophilic sites.
• Compatibility for multiple types of samples: Blood, bone marrow, spleen, peritoneum, and many others.
• Strict quality control ensuring that every data is traceable to its source.
• One-stop services.

Data Support

DATA: Based on Gimesa-Wright staining and lysosome staining, the research identified the differences among macrophages from peritoneal, bone marrow, and splenic. The results displayed that peritoneal macrophages (PMs) had larger cell sizes than bone marrow macrophages (BMs) and splenic macrophages (SPMs). Furthermore, compared to PMs and BMs, SPMs had a longer spindle form and less lysosomal content while PMs and SPMs had more cytoplasm than BMs. These results intuitively reflected the maturity of macrophages from these three sources.

Fig.4 Morphological characteristics of macrophages from peritoneal, bone marrow, and splenic.²

As a leading company in macrophage characterization, Creative Biolabs is constantly delivering helpful technologies to serve global customers. If you want to know more about our morphological difference analysis service by Gimesa-Wright staining and lysosome staining, please get in touch with us at your convenience.

References

1. Duan, Zhaojun, and Yunping Luo. "Targeting macrophages in cancer immunotherapy." Signal transduction and targeted therapy 6.1 (2021): 127.
2. Wang, Changqi, et al. "Characterization of murine macrophages from bone marrow, spleen and peritoneum." BMC Immunology 14.1 (2013): 1-10.