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BALBC Mouse Macrophages, Bone Marrow (MTS-0922-JF37)

Q: What are the recommended applications for this cell line?

They are suitable for a wide range of in vitro experiments, including morphological analysis, physiological studies, gene expression profiling (e.g., cytokine analysis after stimulation), and phagocytosis assays.

Datasheet

Overview

Description

This product is isolated from the bone marrow of pathogen-free laboratory BALBC mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

BALBC Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Are these cells immortalized?

Yes. These are bone marrow-derived macrophages from BALB/c mice that have been immortalized with a retrovirus containing v-raf and v-myc oncogenes. This provides you with an unlimited supply of cells for your research.

What are the recommended applications for this cell line?

They are suitable for a wide range of in vitro experiments, including morphological analysis, physiological studies, gene expression profiling (e.g., cytokine analysis after stimulation), and phagocytosis assays.

We've been using them for months, and they are easy to culture and maintain
Perfect for our Th2-related research. We specifically needed a BALB/c background, and these cells were exactly as described. They polarize beautifully towards an M2 phenotype with IL-4.
No contamination issues whatsoever. Highly recommend
Good cell line, high viability on thaw. They are adherent and have the classic macrophage morphology. The datasheet was comprehensive.
The convenience of an immortalized line is a huge advantage
These cells saved us from breeding BALB/c mice just for bone marrow. They work well for our assays. Just be aware that as an immortalized line, their phenotype might drift at very high passage numbers, so we always keep early-passage frozen stocks.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.