CD1 Mouse Macrophages, Bone marrow, 1 x 10^6 (MTS-0922-JF83)

The CD1 bone marrow macrophages supplied are fully differentiated prior to cryopreservation. We culture bone marrow progenitors in macrophage-promoting conditions and confirm macrophage identity and maturity based on morphology and expression of key markers such as F4/80 and CD11b. After thawing, the cells only need a short recovery period in complete medium before they are ready for experiments. There is no need for you to perform extended differentiation protocols, which saves both time and variability.

In most cases, standard tissue-culture treated plastic plates or flasks are sufficient for good adherence and spreading. If you are performing high-resolution imaging or particularly delicate assays, glass coverslips or specialized imaging plates can be used. We generally do not require coating with extracellular matrix proteins; however, if you wish to mimic specific tissue environments, you can apply coatings like collagen or fibronectin, which the cells tolerate well.

Yes, bone-marrow-derived macrophages are highly relevant for studying inflammatory processes linked to bone and joint disease, such as osteoarthritis or rheumatoid arthritis models. You can expose the cells to inflammatory cytokines, immune complexes, or candidate therapeutic agents and measure changes in cytokine profiles, osteoclast-related markers, or signaling pathways.