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129/SvEV Mouse Macrophages (with STAT1 knockout), Bone Marrow (MTS-0922-JF55)

Datasheet

Overview

Description

With signal transducer and activator of transcription 1 (STAT1), this product is isolated from the bone marrow of pathogen-free laboratory 129/SvEV mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

129/SvEV Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What seeding density and culture conditions do you recommend to obtain consistent responses in stimulation assays?

Consistent seeding density is especially important if you plan to perform cytokine release assays, qPCR, or phospho-protein analysis. Keeping the same plate format and medium across experiments will help reduce variability. Our product datasheet provides additional tips for stimulation timing and reagent concentrations.

Can these STAT1 KO cells be polarized into M1 or M2 phenotypes?

These cells can still be exposed to standard M1 (e.g., LPS + IFN-γ) and M2 (e.g., IL-4, IL-13) polarization protocols. However, the STAT1 deletion significantly affects classical M1 polarization downstream of IFN-γ. You will typically observe diminished induction of certain M1 markers, which is exactly what many researchers are interested in quantifying. M2 polarization via IL-4/IL-13 is generally less dependent on STAT1, so those pathways may be less affected.

How stable is this knockout over time in culture?

Because the knockout is germline and not transient, it is stable across passages when cells are cultured under normal conditions. This stability makes the cells highly suitable for longitudinal or multi-step experiments.

We used the 129 SVEV STAT1 KO macrophages in a study on IFN-γ-mediated gene regulation
The loss of STAT1-dependent transcription was very clear in both RNA-seq and qPCR analyses. Having a ready-made knockout model saved us a lot of time compared with generating our own line.
Cell viability after thawing was high, and the cells attached well to standard tissue culture plastics
We performed multiple rounds of stimulation with cytokines and TLR ligands, and the responses were consistent over several experiments. Batch documentation was thorough and matched our own validation data.
We contacted the support team with questions about polarization protocols and were given detailed, practical guidance
Their experience with these cells saved us a lot of optimization time. From ordering to data generation, the process was smooth and efficient.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.