Online Inquiry

C57BL/6 Mouse Macrophages (with Luciferase expression), Bone Marrow (MTS-0922-JF31)

Q: What is the main advantage of using luciferase-expressing macrophages?

The primary advantage is for in vivo adoptive transfer studies. You can inject these cells into a mouse and then track their migration, proliferation, and localization non-invasively using Bioluminescence Imaging (BLI). This is much more quantitative than fluorescence.

Datasheet

Overview

Description

This product is isolated from the bone marrow of pathogen-free laboratory C57BL/6 mouse. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

C57BL/6 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do I use these cells for in vivo imaging?

After you have adoptively transferred the cells, you will inject the mouse with D-luciferin, the substrate for the luciferase enzyme. The cells will then emit light, which can be captured and quantified using an IVIS or similar BLI system.

What is the main advantage of using luciferase-expressing macrophages?

The primary advantage is for in vivo adoptive transfer studies. You can inject these cells into a mouse and then track their migration, proliferation, and localization non-invasively using Bioluminescence Imaging (BLI). This is much more quantitative than fluorescence.

The signal was incredibly strong and clean
We used these to track inflammation in a peritonitis model. The signal was clear and localized exactly where we expected. Viability was excellent.
This saved us the time and expense of creating our own luciferase-transgenic mouse line
We confirmed they polarize to M1/M2 normally, so they are functionally sound. The luciferase expression is just a bonus for tracking. Will be ordering more.
BLI signal was bright and consistent
The signal-to-noise ratio in our BLI was fantastic. The cell count was on the lower end, but the quality made up for it.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.