Online Inquiry

129 Mouse Macrophages (with IFNGR knockout), Bone Marrow (MTS-0922-JF46)

Datasheet

Overview

Description

With IFN-γ receptor (IFNGR) knockout, this product is isolated from the bone marrow of pathogen-free laboratory 129 mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

129 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

To be clear, is this an IFN-gamma gene knockout or an IFN-gamma receptor knockout?

This is an IFN-gamma receptor (IFNGR) knockout. This means these cells can still produce IFN-gamma if stimulated to do so, but they are completely unable to respond to it (either from an external source or from themselves via an autocrine loop). This is a critical distinction from an Ifng gene knockout.

What are the key differences between this product (MTS-0922-JF46) and the IFN-receptor knockout (MTS-0922-JF44)?

That's an excellent question. This product (JF46) is an IFN-gamma receptor (Type II IFN) knockout. The other product (JF44) is an IFN-alpha/beta receptor (Type I IFN) knockout. They are essentially complementary tools. Use this one (JF46) if you want to study cellular processes in the absence of IFN-gamma signaling. Use JF44 if you want to study processes in the absence of Type I IFN signaling.

What mouse strain are these cells from?

These macrophages are derived from the bone marrow of 129 mice. This 129 genetic background is important for consistency if your other models or cell lines are also 129-based.

Perfect control cells for our lab
We treated these with a high dose of IFN-gamma and saw zero induction of iNOS, while our wild-type 129 cells responded strongly. The KO is legitimate. Great viability and clean culture.
The cells are robust, and the data is unambiguous
These 129-background IFNGR KO macrophages are a great addition to our cell bank. They are much easier than constantly isolating BMDMs from our KO mouse colony. They grow well, though they seem to prefer the recommended medium over our standard DMEM formula.
A good, solid IFNGR KO macrophage line on a 129 background
Used these in parallel with the IFNAR KO line. Creative Biolabs has an excellent set of tools here. The cells arrived quickly on dry ice, thawed with>90% viability, and were CD11b positive as stated.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.