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C57BL/6J Mouse Macrophages, Bone Marrow (MTS-0922-JF41)

Datasheet

Overview

Description

This product is isolated from the bone marrow of pathogen-free laboratory C57BL/6J mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

C57BL/6J Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Are these cells difficult to culture?

Not at all. They are very robust. We provide a recommended medium and handling protocol in the datasheet, and we guarantee the cells will culture well if these instructions are followed.

What are the storage instructions?

The vial is shipped frozen on dry ice. To ensure the highest cell viability for your experiments, you must store it in liquid nitrogen (in the vapor or liquid phase) at or below -150°C upon arrival.

What is the difference between this C57BL/6J line and the C57BL/6 line (JF40)?

The C57BL/6J substrain is the most commonly used inbred mouse strain. However, it has known genetic differences from other substrains (like C57BL/6N), such as a deletion in the Nnt gene. If your research or knockout model is specifically on the 'J' background, using these C57BL/6J macrophages is crucial for the most accurate control.

The genetic background matters!
Our entire knockout colony is on the C57BL/6J background, so we must use C57BL/6J controls. These immortalized macrophages were a perfect find. They behave as expected and are a precise control for our work.
It's nice to have the C57BL/6J substrain specified and available
We use these for all our general macrophage experiments. The fact that they are the 'J' substrain is the standard for our field.
They respond well to M1 and M2 polarization stimuli
Arrived quickly and well-frozen. Thawed with high viability. These are a great alternative to primary BMDMs.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.