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129 Mouse Macrophages (with STAT2 knockout), Bone Marrow (MTS-0922-JF56)

Datasheet

Overview

Description

With signal transducer and activator of transcription-2 (STAT2) knockout, this product is isolated from the bone marrow of pathogen-free laboratory 129 mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

129 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

For which types of projects are STAT2 KO macrophages most commonly used?

STAT2 KO macrophages are particularly valuable for projects focusing on type I interferon pathways, antiviral defenses, and innate immune regulation. They are often used side-by-side with STAT1 KO models to dissect the relative contribution of STAT1 and STAT2 to ISG expression and antiviral activity.

How many passages do you recommend after thawing to maintain physiological relevance and stable phenotype?

We generally recommend limiting these macrophages to 2-3 passages after initial recovery. This helps maintain a phenotype that is close to primary cells and reduces the risk of culture-induced drift. The knockout itself is stable, but extended passaging can influence gene expression and functional responses in any primary-like cell system.

Can I request customized formats?

Yes, we can often accommodate customized formats depending on the project requirements. Some customers request pre-plated cells in multiwell plates for imaging-based assays or compound screening. Others need specific cell numbers per vial or additional QC parameters. If you share details about your experimental platform and throughput, we can propose a tailored solution.

The QC documentation that came with our order was very reassuring
Mycoplasma tests were negative, and the viability data were consistent with what we observed after thawing. The cells attached nicely and showed robust responses to stimuli other than type I IFN, as expected.
We followed the supplier's thawing protocol and had no difficulty establishing the cultures
The cells displayed typical macrophage morphology and tolerated standard manipulations like medium changes and stimulation. We didn't need extensive optimization, which was a big advantage for our small team.
We were interested in using these cells in a high-content imaging assay
The team was open to discussing custom options and provided realistic timelines and pricing. Even though we ultimately chose to plate in-house, the consultation was very helpful.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.