Online Inquiry

CD1 Mouse Macrophages, Liver, 1-10 x 10^6 (MTS-0922-JF60)

Datasheet

Overview

Description

This product is isolated from the liver of pathogen-free laboratory CD1 mouse. 1-10 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

CD1 Mouse Macrophages

Specification

Cell Source

Liver

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 x 10^6 cells, 2 x 10^6 cells, 5 x 10^6 cells, 10 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What isolation methods do you use to enrich liver-resident macrophages (Kupffer cells) from CD1 mice?

We use a multi-step isolation process involving perfusion, enzymatic digestion, gradient centrifugation, and CD11b-based enrichment. This workflow yields a high proportion of Kupffer cells, characterized by distinct morphology and high F4/80/CD68 expression.

Are these liver macrophages suitable for hepatotoxicity and metabolic inflammation studies?

Absolutely. Kupffer cells play a central role in liver inflammation, fibrosis, steatosis, and toxin responses. These CD1 liver macrophages are frequently used in NAFLD/NASH models, LPS-induced inflammatory studies, and xenobiotic metabolism research.

Do you provide recommended assays or marker panels for Kupffer cell characterization?

Yes, we can provide suggested marker panels for flow cytometry, cytokine profiling recommendations, and protocols tailored for hepatotoxicity or metabolic studies. Our technical team can also help optimize culture conditions depending on your planned assays.

We appreciated the purity and viability of this product
We needed a reliable Kupffer cell source for NAFLD studies, and these CD1 liver macrophages exceeded expectations. The liver-specific immune responses were very pronounced, exactly what we needed.
Great performance in hepatotoxicity assays
We used these cells to screen small molecules for liver toxicity. Their cytokine responses were strong and reproducible. They handled repeated stimulations well.
The cells revived quickly after thawing
The QC report included flow data and images that were extremely helpful. Customer support guided us on optimizing our hepatocyte co-culture system.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.