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C57BL/6 Mouse Macrophages (with IFN-gamma knockout), Bone Marrow (MTS-0922-JF45)

Datasheet

Overview

Description

With interferon-gamma (IFN-gamma) knockout, this product is isolated from the bone marrow of pathogen-free laboratory C57BL/6 mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

C57BL/6 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the specific gene knockout in this cell line?

This cell line has a knockout of the Ifng gene, meaning these cells are incapable of producing interferon-gamma (IFN-gamma). This is different from an IFN-gamma receptor knockout; these cells can still respond to exogenous IFN-gamma, but they cannot produce it themselves.

How can I use these cells in my research?

These Ifng knockout macrophages are an exceptional tool for immunology and infectious disease research. They are perfect for co-culture experiments (e.g., with T-cells or other immune cells) to determine the specific contribution of macrophage-derived IFN-gamma to the overall immune response, such as M1 polarization or pathogen clearance.

Do these cells still respond to other stimuli like LPS or Type I interferons?

Yes. The knockout is specific to the Ifng gene. These cells retain a functional innate immune system and will respond normally to other stimuli. For example, they will still respond to LPS via TLR4 and to Type I interferons (IFN-α/β) via the IFNAR receptor, allowing you to study these pathways in the complete absence of autocrine or paracrine IFN-gamma production.

Solid C57BL/6 IFN-gamma KO line
We were struggling with T-cell co-cultures, trying to parse out the source of IFN-gamma. These macrophages completely solved the problem. They grow beautifully and, as expected, produce zero IFN-gamma upon stimulation.
Creative Biolabs delivers again
They respond well to LPS in terms of TNF-alpha production, but obviously, no downstream IFN-gamma-dependent markers. This is exactly the control we needed for our M1 polarization assay. Good viability.
The cells are clean (mycoplasma negative) and easy to work with
They are immortalized, so they are much more robust than primary cells. We confirmed the knockout via ELISA (no IFN-gamma in the supernatant after classic M1 stim). Shipping was fast.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.