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Vessel-associated Macrophages, Skin and Oral Mucosa (MT-0224-HM21)

Datasheet

Overview

Description

Vessel-associated macrophages (VAMs) are a specialized subset of macrophages found in close proximity to blood vessels and lymphatic vessels within various tissues and organs throughout the body. Their distinctive localization allows them to interact with endothelial cells, pericytes, and other components of the vascular wall, contributing to vascular homeostasis, immune surveillance, and tissue integrity.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

Tissue-specific Macrophages

Specification

Cell Source

Skin and Oral Mucosa

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1×10⁶ cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Storage

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can these cells be implemented in 3D vascularized skin or mucosal tissue models?

Absolutely. Vessel-associated macrophages are well suited for incorporation into engineered tissues or organotypic cultures containing vascular structures. Within such systems, they contribute to vessel maintenance, inflammatory signaling, and tissue remodeling. We can share general guidelines on seeding densities, timing of macrophage addition, and media supplements to support successful integration and sustained functionality.

Are the macrophages compatible with high-content imaging or live-cell microscopy workflows investigating endothelial interactions?

Yes. They exhibit stable adherence and morphology under standard imaging conditions, making them suitable for both endpoint and live-cell imaging. Their interactions with endothelial monolayers or microvessel structures can be visualized over time, allowing you to monitor migration, contact formation, and dynamic changes in response to inflammatory or angiogenic stimuli.

Can I order larger quantities or repeated lots for extended vascular research projects?

Yes. We provide options for large-scale production and lot reservation, allowing you to secure sufficient material for multi-phase or multi-site projects. This helps minimize variability and simplifies data comparison across extended timelines. Our team can coordinate production planning and shipping to align with your experimental schedule and capacity.

Overall, the product added significant value to our vascular research platform
We incorporated vessel-associated macrophages into a 3D vascularized skin model and saw a clear improvement in biological relevance compared with endothelial-only setups. The cells participated in angiogenic processes and responded strongly to inflammatory cues, which allowed us to generate more nuanced data on candidate anti-inflammatory compounds.
The macrophages displayed robust adhesion
We could visualize close contacts and track how inflammatory stimuli changed their behavior. Viability remained high throughout the imaging period, and the QC information provided with the shipment helped us understand their baseline characteristics.
Batch-to-batch consistency was strong, which is crucial for our longitudinal comparative studies
Our lab focuses on microvascular inflammation in oral mucosa, and these cells turned out to be an excellent tool. They secreted a distinct cytokine profile in response to pathogen-associated stimuli and supported endothelial activation in a reproducible manner.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.