C57BL/6 Mouse Macrophages, Spleen, 1-10 x 10^6 (MTS-0922-JF79)

Yes, these cells are widely used for polarization studies and respond predictably to standard cytokine cocktails. M1-like polarization can be induced with LPS and IFN-γ, leading to increased expression of genes such as iNOS and elevated secretion of pro-inflammatory cytokines. M2-like states can be promoted with IL-4 or IL-13, resulting in higher arginase-1 expression and production of anti-inflammatory mediators.

Spleen tissue naturally contains a high proportion of lymphocytes, so careful enrichment steps are critical. Our processing workflow includes mechanical dissociation, selective adherence, and macrophage-focused enrichment strategies, followed by flow cytometry to confirm macrophage purity based on markers such as F4/80 and CD11b. Non-adherent cells are removed through wash steps, and the final product is highly enriched for macrophages.

These C57BL/6 spleen macrophages are well suited for imaging-based applications. They adhere strongly to tissue-culture treated plates or glass, making them compatible with high-content imaging platforms and live-cell microscopy. Their clear morphology and ability to internalize fluorescent cargo make them ideal for visualizing phagocytosis, intracellular trafficking, or signaling events. We recommend using appropriate fluorophore combinations and including proper controls.