Lung interstitial macrophages (LYVE1^lowMHCII^high), Lung (MT-0224-HM16)

We employ stringent gating strategies on multiparameter flow cytometry, assessing Lyve1, MHCII, and a panel of macrophage and lineage markers to exclude unwanted populations. Additional checks include viability, morphology, and selected transcriptional or cytokine signatures. Only preparations with high purity and consistent Lyve1^low MHCII^high profiles are approved for release, and QC data are summarized in a batch-specific report provided to each customer.

Yes. They can be combined with dendritic cells, T cells, or other immune populations in 2D or 3D co-culture systems. Their antigen-presentation capacity and cytokine output complement that of other APC types, allowing more complex modeling of the pulmonary immune environment. We provide general advice on seeding ratios and culture media to support stable, interpretable co-culture experiments.

Yes. For projects that require a defined activation state, we can pre-stimulate the macrophages with specified cytokines or pattern-recognition receptor ligands under controlled conditions. After stimulation, we confirm viability and key activation markers. This service provides ready-to-use, pre-conditioned cells, helping you reduce preparation time and variability in sensitive immunological assays.