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Mouse Macrophages (with sialoadhesin expressing)AA9 (MTS-0922-JF51)

Datasheet

Overview

Description

Mouse macrophage cells with sialoadhesin expression. This product can be used in in-vitro propagation of porcine reproductive and respiratory syndrome virus (PRRSV). It is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. This product is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

Mouse Macrophages

Specification

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

The description mentions PRRSV. What is the connection?

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is known to use sialoadhesin as a receptor for entry into macrophages. This cell line, by expressing sialoadhesin, is a non-porcine (mouse) model that can be used for the in vitro propagation and study of PRRSV, as well as for screening antiviral compounds that target this specific interaction.

Are these cells suitable for research not related to PRRSV?

Definitely. Sialoadhesin-expressing macrophages are important in many other areas of immunology. They are known to be involved in antigen presentation, cell-cell interactions (e.g., with T-cells and B-cells), and the capture of other pathogens (like certain bacteria and viruses) or even exosomes. If your research involves any of these Sialoadhesin-mediated functions, this cell line is ideal.

What is special about this cell line?

This cell line is specifically noted for its expression of Sialoadhesin (also known as Siglec-1 or CD169). Sialoadhesin is a macrophage-restricted adhesion molecule that binds to sialic acids. This product (AA9) is a specific clone or line selected for this expression, making it a valuable tool for studying Sialoadhesin-mediated interactions.

So glad to have this immortalized tool
We use these cells exclusively for our PRRSV work. They are highly permissive to the virus and give very consistent titers. Far more reliable and easier to handle than primary porcine alveolar macrophages.
A good, stable line for viral work
We are using it as a positive control for sialoadhesin-mediated viral entry. Viability was excellent upon thawing. They grow a bit slower than RAW cells, but that's to be expected. Solid product.
Shipping was fast and the cells were well-packaged on dry ice
I'm not in a virology lab; I study B-cell/macrophage interactions in germinal centers. This cell line is an excellent in vitro model for CD169+ macrophages. They perform beautifully in our co-culture assays.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.