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Macrophage Detachment Solution (MTS-1223-HM4)

Datasheet

Overview

Description

Creative Biolabs offers chemically defined, non-enzymatic detachment solution optimized for gentle detachment of strongly adherent macrophages.

Applications

Cell Culture

Product Type

Reagent

Specification

Quality Control

Tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Size

250 mL

Storage

2-8°C

Notes

This prodcut is not suitable for the dissociation of other adherent cell types.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.W6:W9

FAQs Customer Reviews Related Products

Is this detachment solution gentle enough for sensitive primary macrophages without damaging surface markers?

Yes. The Macrophage Detachment Solution is specifically formulated to detach adherent macrophages with minimal impact on cell viability or surface antigen expression. It avoids harsh proteolytic activity typically associated with standard trypsinization. After treatment, most cells retain intact markers suitable for flow cytometry, phenotyping, or downstream functional assays, making it ideal for primary and delicate macrophage cultures.

Will residual solution interfere with downstream assays such as cytokine measurement or functional studies?

When used as directed, the solution is easily removed by washing the cells with fresh culture medium after detachment. Residual amounts are generally negligible and do not interfere with typical assays, including cytokine ELISAs, flow cytometry, phagocytosis assays, or gene-expression analysis. We recommend a brief wash step to ensure optimal assay performance.

Does the solution require neutralization or special handling after use?

No special neutralization step is required. After detachment, simply dilute and wash the cells with fresh culture medium and proceed with your usual centrifugation and resuspension steps. Standard laboratory safety and handling precautions apply, and aseptic technique should be maintained to avoid contamination.

It has become a reliable part of our routine cell-handling protocol
The solution works well on both short-term and long-term cultures. Even after several days in culture, macrophages detach with minimal effort and recover quickly after reseeding.
It is particularly useful when we need to detach cells for co-culture or transwell assays
We were initially skeptical about switching from our traditional methods, but this product really made a difference. Our recovery yields improved, and functional responses remained strong.
Overall, this is a very well-designed detachment solution
It strikes a good balance between efficiency and gentleness, which is exactly what you want when working with sensitive primary macrophages.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.