Macrophage cells are critical components of the immune response. Macrophage cell-based delivery systems are a promising field in cell therapy. Creative Biolabs, as an experienced cell therapy CRO, can assist in the engineering of macrophage cells loaded with multiple cargos.
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Product Overview
Macrophages are tissue-resident immune cells of the mononuclear phagocyte system, which play a role in both the adaptive and innate immune systems. Tissue Macrophages were either established prior to birth or self-sustaining through bone marrow-derived monocyte recruitment. Macrophage function and phenotype change in response to their microenvironment. They can be transiently polarized into the M1 or M2 phenotypes, with M1 being the classically activated proinflammatory phenotype and M2 being the activated anti-inflammatory type.
Fig. 1 Schematic illustration of the construction of ‘Macrophage-NPs’ and their targeting delivery into brain tumor. (Liang, 2016)
Advantages of Macrophage-Mediated Drug Delivery Systems
Because of their inherent properties, macrophages have a high capacity for delivering cargos to solid tumors. They have the ability to cross biological barriers, penetrate deep into tumors, and accumulate in particularly hypoxic and poorly vascularized areas that are difficult to access. Additionally, Macrophage is an excellent therapeutic cell for re-modulating the suppressive tumor microenvironment.
Live macrophages uptake the drugs or nanoparticles by phagocytosis. Drug-carrying macrophages can escape host immune system surveillance, extending the persistence time and half-life of a drug. Furthermore, macrophages have multiple surface receptors that allow for the targeted delivery of various payloads.
Our Capabilities
Our teams are proficient in a wide range of technologies used in macrophage engineering processes. At Creative Biolabs, macrophages act as Trojan horses to deliver cytokines, drugs, and drug-loaded nanoparticles to TME. Alternatively, they are armed with engineered receptors.
We offer primary macrophages and macrophage cell lines:
Human PBMC-derived macrophages
Human PBMC-derived monocytes
Human monocyte-derived macrophages
Mouse bone marrow-derived macrophages (BMDM)
Mouse bone marrow-derived hematopoietic stem/progenitor cells (HSPCs)
Years of experience in macrophage research allow us to advise clients on the best macrophage products for potent delivery advantage. We provide products tailored to client specifications by combining chemical and cell biology expertise with cutting-edge instrumentation. Please contact us if you want to collaborate with our experts to accelerate your program.
Related Services
In recent years, the applications of macrophages as drug carriers with different properties have been significantly expanded. As a leading specialist in engineering macrophages, Creative Biolabs provides comprehensive R&D services for engineering M1 macrophages, macrophage-derived exosomes, and macrophage membranes as drug carriers.
By leveraging the wealth of information on macrophage engineering, Creative Biolabs is pleased to help our clients with their project development in engineering macrophages as drug carriers. Our multidisciplinary teams work together closely to address these scientific and technical challenges.
Macrophage-derived exosomes are attractive drug delivery vehicles for cancer treatment. Creative Biolabs offers fast, reliable support for engineering macrophage-derived exosomes as drug carriers.
Creative Biolabs is glad to serve our global clients with professionalism and expertise in engineering macrophage membranes as drug carriers.We are well-equipped and versed in engineering macrophage membrane coating with drug or drug-loaded NPs.
Provide efficiencies with shorter timelines
Enhance the drug delivery efficiency
Publications
Tingting Xiao et al. reported a uniquely designed and updated hybrid nanogel (NG) system for effective macrophage-mediated tumor delivery and enhanced tumor therapy. The innovation of this study is that the utilization of NG can enhance its loading in macrophages (MAs) through a receptor-mediated approach, mitigating the toxic effects on MAs.
It was shown that MAs-NGs retained comparable tumor-targeting ability to MAs and that both MAs and MAs-NGs were predominantly distributed in the tumor, liver, and spleen. The tumor/liver fluorescence ratios in the MAs and MAs-NGs groups were much higher than those in the NGs group. Overall, under MA-mediated tumor homing, NGs could easily escape from the reticuloendothelial system, thus improving the tumor therapeutic efficacy.
Fig. 2 In vivo fluorescence images of mice at different time points after injection of NGs, MAs and MAs-NGs.2
Applications
Our products offer innovative applications across various research domains.
Targeted Drug Delivery
Vaccine Development
Gene Therapy
Study of Macrophage Biology
Assessment of Drug Efficacy and Toxicity
Biomarker Discovery
Our macrophage-mediated delivery system products provide versatile applications that enhance research capabilities in drug development, immunology, regenerative medicine, and beyond.
Q: Can this system target specific tissues or cells?
A: Yes, the macrophage-mediated delivery system is designed to target specific tissues or cells. By modifying the surface properties of the macrophages, we can enhance their targeting capabilities. Our team offers advice on modifying targeting ligands or markers to align with your research goals and improve specificity.
Q: How do you ensure product quality and reproducibility?
A: We maintain stringent quality control measures throughout the manufacturing process of our macrophage-mediated delivery systems. Each batch undergoes rigorous analysis for consistency and efficacy using state-of-the-art techniques. In addition, we conduct reproducibility studies to validate the performance of our systems in different research scenarios. Detailed quality assurance documentation is available to our customers, ensuring confidence in the integrity and reliability of our products.
Q: What is the shelf life of macrophage-mediated delivery products? How should they be stored?
A: The shelf life of macrophage-mediated delivery products depends on the specific formulation and storage conditions. Typically, products should be stored at -20°C or -80°C to maintain stability and efficacy over time. Detailed storage guidelines are included with each product to ensure they remain viable for the intended research applications, minimizing degradation and preserving functional activity.
Q: Can I use macrophage-mediated systems in both in vitro and in vivo studies?
A: Yes, macrophage-mediated delivery systems are versatile and can be used in both in vitro and in vivo studies. In vitro, they enable precise control over conditions to study drug delivery mechanisms, cellular interactions, and immune responses. For in vivo studies, macrophages' natural migratory capabilities allow them to localize to disease sites, making them suitable for preclinical animal models to assess therapeutic efficacy, biodistribution, and immune modulation in a live system.
Customer Reviews
An excellent model for studying immune system interactions
It's very straightforward to set up, and macrophage loading was faster and more efficient than expected. The macrophages integrated smoothly, delivering our payloads without disturbing native cell functions.
United States
08-22-2024
This system has really streamlined our workflow in research
We used this for RNA delivery in our recent study, and the macrophages mediated the transfer with minimal immune reaction. Our experiments have become much more efficient.
United States
08-25-2024
Our lab used this product
The delivery system was easy to implement. We noticed a significant reduction in off-target effects but didn't observed consistent delivery efficiency.
United States
08-29-2024
References
Liang, P.; et al. Exploiting macrophages as targeted carrier to guide nanoparticles into glioma. Oncotarget vol. 7,24 (2016): 37081-37091.
Xiao, Tingting, et al. "Macrophage-mediated tumor homing of hyaluronic acid nanogels loaded with polypyrrole and anticancer drug for targeted combinational photothermo-chemotherapy." Theranostics 11.14 (2021): 7057, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/).
Fig. 1 Schematic illustration of the construction of ‘Macrophage-NPs’ and their targeting delivery into brain tumor. (Liang, 2016)
Fig. 2 In vivo fluorescence images of mice at different time points after injection of NGs, MAs and MAs-NGs.2
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