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MacroCargo™ Human Monocyte-derived Macrophages (MDMs) with PD-L1 gRNA (Viral System, Lentivirus) (MTS-1122-YF47)

Cell Properties

Product Overview
As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Human Monocyte-derived Macrophages (MDMs) carried with PD-L1 gRNA by Viral System-Lentivirus. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.
Cell Name
Monocyte-derived Macrophages (MDMs)
Cell Type
Primary Cell
Cell Species
Human
Cell Background
The culture of human monocyte-derived macrophages (MDMs) represents a tool to study macrophages, with monocytes known to give rise to tissue macrophages influenced by certain environmental cues.

Cargo Properties

Cargo Type
CRISPR KO
Specific Cargo
PD-L1 gRNA
Target Common Name
CD274
Target Alternative Names
B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1
Target Full Name
CD274 molecule
Introduction
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
UniprotID
GeneID
Cargo Delivery System Type
Viral System
Cargo Delivery Approach
Lentivirus

Product Properties

Applications
Resistant to reprogramming by tumor-secreted signals
References
Moyes, Kara W et al. "Genetically Engineered Macrophages: A Potential Platform for Cancer Immunotherapy." Human gene therapy vol. 28,2 (2017): 200-215. doi:10.1089/hum.2016.060
Mycoplasma Testing
Negative
Sterility Testing
Negative
Shipping
Dry ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Restriction
Research use only

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.

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