U937 cells are commonly used in biomedical research, particularly in studies related to immunology, inflammation, cancer, and infectious diseases. They serve as a convenient model for investigating monocyte/macrophage biology, differentiation, and function in vitro. Creative Biolabs develops U937-based macrophage models to provide quality-controlled cell models and characterization services for your biomedical research.

U937 Cell

U937 cells are a human leukemic monocyte lymphoma cell line that was derived from the pleural effusion of a patient with histiocytic lymphoma. These cells exhibit properties of monocytes and can be differentiated into macrophage-like cells in response to various stimuli, such as phorbol esters like phorbol myristate acetate (PMA).

U937-based Macrophage Model Development Service at Creative Biolabs

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Display Results

Functional Assays
Fig.1 The effect of differentiation with PMA, IFN-c or VD3 on the ability of U937 cells to bind Mycobacterium tuberculosis.1	The ability of U937 cells to bind Mycobacterium tuberculosis in the presence of fresh human serum under different conditions was investigated using ZN staining and light microscopy.
Fig.2 The effect of differentiation with PMA, IFN-c or VD3 on the ability of U937 cells to phagocytose Mycobacterium tuberculosis.1	The left panel shows Mycobacterium tuberculosis contained in PMA-differentiated U937 cell intima-bound phagosomes by transmission electron microscopy.

Frequently Asked Questions

Q1: Why choose the U937 macrophage model?

A1: The U937 macrophage model offers a valuable tool for studying human macrophage biology, immune responses, inflammation, and diseases in vitro, with advantages including human relevance, differentiation potential, versatility, consistency, ease of culture, and genetic manipulability.

• U937 cells are derived from human leukemic monocytes, making them more physiologically relevant for studying human immune responses compared to murine cell lines.
• U937 cells can be induced to differentiate into macrophage-like cells in vitro by treatment with agents like phorbol esters (e.g., PMA).
• After differentiating, U937-derived macrophages exhibit characteristics similar to primary macrophages, including phagocytic activity, cytokine production, and antigen presentation.
• Being a cell line, U937 maintains their phenotype and behavior over multiple passages, reducing variability in experimental results compared to primary cells.

Creative Biolabs has expertise and scientists in the field of cell culture, model development, and related macrophage research. We provide you with high-quality and reliable results. If you need U937 macrophage model, you can contact us at any time. Our scientists are available around the clock.