Tissue-specific Macrophage Immortalization Service

Macrophages are an essential part of the immune system, playing a key role in phagocytosis of pathogens and maintaining homeostasis in the body. However, these cells do not replicate easily in culture, making it challenging to study them over long periods of time. Immortalization is a process that involves altering the genetic makeup of cells to prevent them from aging and dying, allowing for unlimited growth and proliferation in vitro. Therefore, immortalizing macrophages is of great significance in the study of disease diagnosis and treatment. Creative Biolabs offers a specialized service for immortalizing macrophages from specific tissues, providing researchers with high-performing cell lines tailored to their needs.

Fig.1 Comparison of primary (A) and immortalized (B) porcine kidney macrophages in culture. (Takenouchi, et al., 2017)Fig.1 Comparison of the physical appearance of primary (A) and immortalized (B) porcine kidney-derived macrophages in culture.1

Tissue-specific Macrophage Immortalization Service at Creative Biolabs

Creative Biolabs' tissue-specific macrophage immortalization service utilizes two distinct mechanisms for immortalization. Firstly, Inhibition of exogenous viral protein or induction of self-mutation triggers the activation of proto-oncogenes or inactivation of tumor suppressor genes. This enables tissue-specific macrophages to circumvent the normal cellular checkpoints, thereby blocking pathways that lead to cell aging and death, and facilitating infinite proliferation. Secondly, we activate telomerase to extend the length of telomeres, ensuring continued cell proliferation of tissue-specific macrophages.

Furthermore, we are able to customize specific immortalization methods for macrophages from different tissues. In addition to that, we also have the ability to characterize these immortalized tissue-specific macrophages according to their specific markers and your intended applications. Together with our experienced scientists, Creative Biolabs is confident in presenting excellent-performed immortalized tissue-specific macrophages in a short turnaround.

Exogenous Viral Protein for Tissue-Specific Macrophage Immortalization Service

  • SV40 virus
  • Epstein-Barr virus
  • human papillomavirus HPV

The Advantage of Tissue-specific Macrophage Immortalization

  • Immortalized tissue-specific macrophage resources improve the plight of less macrophage passage and slow proliferation so that tissue-specific macrophage can be infinite passage.
  • The immortalized tissue-specific macrophage growth is more robust, saving the experiment cycle.
  • Immortalized tissue-specific macrophage is a good model for research on cancer treatment and inhibition of cancer cell proliferation.
  • Genetically, immortalized tissue-specific macrophages belong to the same population, we are able to establish a cell bank with the same genetic traits, which contributes to the stability of experimental results.

Frequently Asked Question

Q: What are the requirements for samples?

A: To immortalize tissue-specific macrophages, it is necessary to ensure adequate cell samples with a concentration exceeding 106 cells per tube for optimal immortalization and screening of high-quality macrophages. In cases where tissue-specific macrophage samples have not been isolated, our services can assist in the isolation, culture, and immortalization processes, requiring ample fresh tissue samples. For projects with special needs, please contact our professional team for assistance.

If you want to know more details about our tissue-specific macrophage immortalization service, please don't hesitate to reach out to us.


  1. Takenouchi, Takato, et al. "Immortalization and characterization of porcine macrophages that had been transduced with lentiviral vectors encoding the SV40 large T antigen and porcine telomerase reverse transcriptase." Frontiers in Veterinary Science 4 (2017): 132.
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