Microglia and CNS-associated Macrophage Analysis Services
Microglia and CNS-associated macrophages are specialized immune cells that play crucial roles in maintaining the health and function of the CNS. They are associated with almost all diseases of the central nervous system. However, it is difficult to phenotypically distinguish inflammatory microglia from tumor-lesioned associated microglia, as well as monocytes recruited from the periphery and differentiated into MdCs. Therefore, more investigation is required to ascertain the functional consequences of these changes in relation to the disease. Based on our macrophage therapeutic platform, Creative Biolabs delivers a one-stop service to characterize microglia and CNS-associated macrophages under various contexts. We encourage global customers to inquire and place orders.
Fig.1 Possible mechanism of CNS-associated macrophages in PD.1
Microglia and CNS-associated Macrophage Analysis Service at Creative Biolabs
Creative Biolabs' microglia and CNS-associated macrophage analysis service incorporates expertise in cell biology and advanced analysis technologies. Based on this, we deliver a wide range of approaches such as immunostaining, flow cytometry, and molecular biology techniques to multidimensionally analyze macroglia and CNS-associated macrophages. We also offer assays to analyze macroglia and CNS-associated macrophages including electron microscopy analysis, reactivity analysis, neurotoxic factor release analysis, and pathological function analysis, which is useful for their function elicitation in CNS. Moreover, we have the ability to isolate and culture microglia and CNS-associated macrophages. Through stimulating them in vitro, we are able to model their reaction and mechanism under the pathological complex of the CNS. Collectively, our seasoned research team will help every customer make the appropriate experiment and obtain the desired outcomes in a short turnaround.
Several Approaches Available at Creative Biolabs
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Immunohistochemical staining or immunofluorescence staining: Capable of displaying and locating microglia and CNS-associated macrophages. Specific antibodies are used to mark specific markers on them, which can clearly show their morphology and distribution.
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Flow cytometry: Capable of distinguishing and counting microglia and CNS-associated macrophages, as well as multi-parameter analyzing single macrophage.
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Molecular biology techniques: Encompassing real-time qPCR, gene chip, etc., these techniques have the potential to analyze the gene expression profiles of these macrophages and allow insight into their functional changes under different physiological and pathological conditions.
Specific Markers for Microglia and CNS-associated Macrophage Analysis
Marker is crucial for characterizing microglia and CNS-associated macrophages. Creative Biolabs provides a bank of well-identified specific markers to design for customers' projects:
Fig.2 Specific Markers.
Advantages
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Fast timelines.
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Multidimensional analysis.
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Numerous specific markers are available.
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Well-developed analysis approaches supporting.
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Comprehensive Analysis services.
Frequently Asked Question
Q1: What is the difference between microglia and CNS-associated macrophages?
A1: Tissue macrophages in the CNS include microglia in brain tissue and CNS-associated macrophages in interfaces like meninges and choroid plexus. CAMs, often referred to as border-associated macrophages (BAMs), include choroid plexus macrophages, subdural leptomeningeal macrophages (MMs), and perivascular macrophages (PVM). Microglial cells are highly conserved across evolution, indicating that their functions are likely similar across different species.
For more details about our microglia and CNS-associated macrophage analysis service, please don't hesitate to get in touch with us.
Reference
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Frosch, Maximilian, Lukas Amann, and Marco Prinz. "CNS-associated macrophages shape the inflammatory response in a mouse model of Parkinson's disease." Nature Communications 14.1 (2023): 3753.
