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C57/129 Mouse Macrophages, Bone Marrow (MTS-0922-JF52)

Datasheet

Overview

Description

This product is isolated from the bone marrow of pathogen-free laboratory C57/129 mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

C57/129 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What does "C57/129" mean? Is this a mixed strain?

"C57/129" indicates that this cell line is derived from a mouse with a mixed genetic background of C57BL/6 and 129 strains. This is very common in research, as many knockout mice are created by targeting embryonic stem cells from a 129 strain and then backcrossing them (often incompletely) onto a C57BL/6 background.

Are these cells suitable for general macrophage studies?

Yes, they are perfectly suitable for a wide range of studies, including morphological analysis, gene expression studies (e.g., M1/M2 polarization), and physiological assays (e.g., phagocytosis). They are particularly valuable, however, as the genetically-matched wild-type control for experiments involving C57/129-background knockout cells.

Why would I choose a mixed background cell line?

This cell line is the ideal "control" for many common knockout mouse lines. If your knockout mouse has a mixed C57/129 background, it is crucial to use a control macrophage line with the same mixed background. Using a pure C57BL/6 or pure 129 line could introduce genetic variables that confound your results.

This is the control line we've been searching for
Our knockout mouse colony is on a mixed B6/129 background, and finding a matching immortalized macrophage line was difficult. These are perfect. They grow well and are a solid baseline for our experiments.
A good, robust, 'workhorse' macrophage line
We bought these to compare against our pure C57BL/6 line, and we can already see interesting differences in gene expression. The mixed background is a valuable tool in itself. Cells are clean and viability was>95%.
This is a very useful control line to have in the freezer.
A C57/129 wild-type macrophage line. We use this as the control for our knockout lines (also from Creative Biolabs), and the data is very clean. It's great to have a single supplier for all our matched cell lines.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.