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B129 Mouse Macrophages, Bone Marrow, 6 x 10^6 (MTS-0922-JF23)

Datasheet

Overview

Description

This product is isolated from the bone marrow of pathogen-free laboratory B129 mouse. 6 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

B129 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

6 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the specific application for the B129 mouse strain? Why would I choose this over C57BL/6?

The B129 (or 129) mouse strain is a common genetic background used for creating knockout (KO) models, as many embryonic stem (ES) cell lines are derived from it. You should choose these B129-derived macrophages if your in vivo study uses KO mice on a 129 background, as these cells are the genetically appropriate in vitro control.

What is the total cell count and expected viability?

This product contains a guaranteed 6 x 10^6 (6 million) viable cells per cryovial. We guarantee high viability upon thawing, provided our recommended thawing protocol and culture medium are used.

What is the recommended medium for culturing these BMDMs?

We provide a detailed protocol with your order. Generally, BMDMs thrive in DMEM or RPMI-1640 supplemented with 10% Fetal Bovine Serum (FBS) and M-CSF to maintain their viability and differentiation.

No contamination, high purity. Will buy again
We needed the 129 background for our in vivo comparison. They behaved as expected. Viability was about 85-90% post-thaw, which is good. Happy with the purchase.
The cells were highly viable and polarized to M1 just as well as B6 cells
Saved us the hassle of sacrificing our 129 mice just for BMDMs. This is much more convenient and cost-effective. Quality is top-notch.
The 6 million cell count is generous
Adhered well and were ready for stimulation after 24 hours. The protocol provided was clear and helpful. A reliable source.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.