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Mouse Macrophages (with NFKB p50 knockout), Bone Marrow (MTS-0922-JF53)

Datasheet

Overview

Description

With nuclear factor kappa-light-chain-enhancer of activated B cells subunit 50 (NF-κB p50) knockout, this product is isolated from the bone marrow of pathogen-free laboratory mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How will these cells respond to stimuli like LPS?

The response will be significantly altered, which is the purpose of this line. LPS strongly activates the canonical NF-κB pathway. In these p50 KO cells, the p50/p65 activator cannot be formed. This often leads to a dampened or altered pro-inflammatory response for many genes. However, the loss of the p50/p50 repressor can also lead to the overexpression of other genes. This cell line is the key to identifying which genes are controlled by which NF-κB dimer.

Are these cells suitable for studying the non-canonical NF-κB pathway?

Yes, they are an excellent tool for that. The canonical pathway is disrupted, but the non-canonical pathway (which relies on p52/RelB) is left intact. This allows you to study non-canonical NF-κB activation in isolation, without the dominant, overlapping effects of the p50/p65 canonical pathway.

What mouse strain background are these cells?

These macrophages are isolated from pathogen-free laboratory mice with the p50 (Nfkb1) knockout. For specific strain details (e.g., C57BL/6), please contact our technical support team, as this is crucial for interpreting NF-κB biology, which can be strain-dependent.

We confirmed the p50 knockout by Western blot
We are studying the non-canonical NF-kB pathway, and these cells are the perfect background. We can stimulate with non-canonical activators and see a clean p52/RelB-dependent response without canonical pathway interference.
The cells are clean and grow well
We are seeing some very interesting (and unexpected!) phenotypes with these cells, as the p50/p50 repressor function is gone. A great tool for discovery. They just grow a bit slowly.
Solid p50 KO macrophage line
We've used it to show that our drug's effect is p50/p65-dependent, as the drug does nothing in these cells. The data is very clear. Viability was great on arrival.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.