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Rabbit Macrophages, Bone Marrow, 6 x 10^6 (MTS-0922-JF85)

Datasheet

Overview

Description

This product is isolated from the Tibia or femur bone of pathogen-free laboratory rabbit. 6 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Rabbit

Product Type

Rabbit Macrophages

Specification

Cell Source

Tibia or femur bone

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

6 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do these rabbit macrophages perform in bacterial uptake and killing assays?

Rabbit bone marrow macrophages show strong phagocytic capacity toward both Gram-positive and Gram-negative bacteria. They internalize opsonized bacteria efficiently and can generate reactive oxygen and nitrogen species after activation, making them suitable for studying bactericidal functions.

Are there any special considerations when culturing rabbit macrophages compared with rodent macrophages?

While many aspects of culture are similar, rabbit macrophages can sometimes exhibit subtle differences in adherence dynamics and sensitivity to certain serum lots. We recommend using high-quality sera and following the medium and seeding recommendations provided in the product documentation.

Can I combine these rabbit macrophages with autologous or heterologous serum in my experimental design?

Yes, many users incorporate rabbit serum, either autologous or from defined sources, into their experimental setup when studying complement activation, opsonization, or immune complex handling. The macrophages are compatible with such designs, provided that the serum is properly handled and not cytotoxic at the concentrations used. If you plan to introduce sera from other species, we recommend performing small pilot tests to make sure there are no unanticipated effects on cell viability or function.

We used the rabbit macrophages to evaluate complement-related effects of candidate therapeutics
The cells tolerated various serum conditions, and we observed robust, interpretable responses. The product's QC documentation and SPF animal sourcing were important points for us, given the sensitivity of our assays to background activation.
After thawing, they attached reliably to culture plates and maintained typical macrophage morphology over several days
We could perform both imaging-based assays and ELISA-based cytokine measurements without issues. Overall, the product performed as described.
We appreciate that the supplier offers such a specialized product
Rabbit macrophages are not easy to source with this level of quality control. The cells we received have been reliable across multiple experiments, and we plan to keep using them in our ongoing studies.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.