Online Inquiry

Mouse Macrophages (with IGTP knockout), Bone Marrow (MTS-0922-JF47)

Q: How can these IGTP knockout macrophages be used in research?

These cells are an ideal in vitro model for studying cell-autonomous immunity. They are particularly useful for infection studies with intracellular pathogens. By comparing infection progression in these knockout cells versus wild-type macrophages, you can precisely define the role of IGTP and the IRG system in controlling or eliminating the pathogen.

Datasheet

Overview

Description

With interferon gamma-induced GTPase (IGTP) knockout, this product is isolated from the bone marrow of pathogen-free laboratory mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How can these IGTP knockout macrophages be used in research?

These cells are an ideal in vitro model for studying cell-autonomous immunity. They are particularly useful for infection studies with intracellular pathogens. By comparing infection progression in these knockout cells versus wild-type macrophages, you can precisely define the role of IGTP and the IRG system in controlling or eliminating the pathogen.

What is the significance of knocking it out?

IGTP (Interferon Gamma-Induced GTPase) is a member of the p47 family of immunity-related GTPases (IRGs). In mice, these proteins are critical mediators of IFN-gamma-dependent cell-autonomous immunity, particularly against intracellular pathogens like Toxoplasma gondii and Chlamydia. Knocking out IGTP severely cripples this specific defense pathway.

This is a fantastic tool
We confirmed the IGTP knockout at the protein level (no band on Western blot after IFN-g treatment). The cells are clean, grow well, and are saving us the hassle of breeding IGTP KO mice.
Performed as described
These cells are a perfect susceptible host model for our intracellular bacteria studies. The only thing I'd wish for is more explicit strain background info on the product page itself, but tech support was quick to provide it.
I'm very happy with this purchase
The cells are robust for an immortalized line and provide a very clean background for studying the IRG pathway. We've already generated some great data for a manuscript. Will buy again.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.