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C57BL/6 Mouse Macrophages (with DAP12 knockout), Bone Marrow (MTS-0922-JF42)

Datasheet

Overview

Description

With DAP12 knockout, this product is isolated from the bone marrow of pathogen-free laboratory C57BL/6 mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

C57BL/6 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the function of DAP12, and why would I want a knockout line?

DAP12 (also known as TYROBP) is a key signaling adaptor protein that pairs with various activating receptors on immune cells, including macrophages and NK cells. It's involved in inflammation, phagocytosis, and cell activation. A DAP12 knockout macrophage line is an essential tool for studying the specific pathways and receptors that depend on DAP12 signaling.

What control cell line should I use with this product?

We highly recommend using our standard C57BL/6 Bone Marrow Macrophages (Cat# MTS-0922-JF40) as your wild-type control. It is derived from the same mouse strain (C57BL/6) and immortalized using the exact same method.

What is the protocol for thawing and culturing these cells?

A detailed protocol, including recommended culture medium and thawing instructions, is provided on the product datasheet. The cells are shipped frozen on dry ice and must be stored in liquid nitrogen upon arrival.

The cells are healthy, grow well, and the knockout phenotype is stable
We study TREM2 signaling, which is dependent on DAP12. This cell line was exactly what we needed. We've confirmed the lack of DAP12 signaling and the line is working perfectly in our assays.
We are very happy with this DAP12-KO line
They are easy to culture and handle. As expected, their response to stimulation of DAP12-associated receptors is completely blunted. Great data.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.