Online Inquiry

Human Peripheral Blood Macrophages (MTS-1223-HM9)

Q: Can I use these macrophages for pathogen infection models, such as bacteria, viruses, or fungi?

Many clients use these cells for in vitro infection models, as they are robust phagocytes and express pattern recognition receptors relevant to microbial detection.

Datasheet

Overview

Description

Creative Biolabs offers frozen, peripheral blood-derived primary human macrophages.

Applications

Macrophages

Product Type

Cell

Specification

Cell Source

Peripheral Blood

Size

1,500,000 cells

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How are your human peripheral blood macrophages different from pre-polarized M1 or M2 cells, and in what kinds of studies are they most commonly used?

These macrophages are differentiated from peripheral blood monocytes without forcing them into a strongly polarized M1 or M2 state. As such, they represent a more physiologic, tissue-like macrophage population that has completed differentiation but not undergone heavy activation in either direction. They are commonly used for phagocytosis assays, host-pathogen interaction studies, metabolic profiling, and as a baseline for moderate activation experiments.

Can I use these macrophages for pathogen infection models, such as bacteria, viruses, or fungi?

Many clients use these cells for in vitro infection models, as they are robust phagocytes and express pattern recognition receptors relevant to microbial detection.

How suitable are these peripheral blood macrophages for metabolic or transcriptomic profiling studies?

These cells are highly suitable for omics-level analyses because they represent a physiologically relevant differentiated macrophage population. Researchers often use them for transcriptomic profiling to examine gene-expression changes following treatments or for metabolic studies using platforms like Seahorse or stable-isotope tracing. As always, we recommend pilot experiments to optimize cell density, time points, and experimental conditions for your specific readouts.

We have used these peripheral blood macrophages extensively to model host-pathogen interactions in vitro
The cells consistently show strong phagocytic capacity and robust, but not exaggerated, cytokine responses after infection. This gives us a dynamic range that is very useful for testing both pro- and anti-inflammatory interventions. Recovery after thawing has been reliable, which helps us start experiments on schedule without last-minute surprises.
Our metabolic core facility needed a dependable source of primary human macrophages for Seahorse-based assays
These cells adhered well to the assay plates, tolerated the flux measurements, and produced clear metabolic signatures when we introduced different stimuli. The relatively low baseline activation state allowed us to see distinct shifts toward glycolysis or oxidative phosphorylation depending on the treatment. Overall, they fit very well into our workflow.
We are working on a series of experiments looking at how novel biomaterials interact with primary macrophages
These peripheral blood-derived macrophages attached readily to our experimental surfaces and provided measurable changes in morphology and cytokine secretion that helped us rank candidate formulations. The quality of the cells was consistent across several orders, which is crucial for long-term material development.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.