Human M1 Macrophages (GM-CSF), monocyte-derived (MTS-1223-HM6)

Under our recommended culture conditions, these GM-CSF-derived M1 macrophages usually maintain a stable pro-inflammatory phenotype for at least 5-7 days after thawing. During this period, they retain characteristic M1 morphology and surface-marker expression and continue to secrete pro-inflammatory cytokines upon appropriate stimuli. To minimize phenotype drift, we advise against prolonged culture beyond one week, repeated passaging, or exposure to high concentrations of anti-inflammatory agents such as IL-4 or glucocorticoids unless such transitions are part of your experimental design. Our technical support team can also suggest specific culture and stimulation timelines tailored to your assay endpoints.

Yes, these M1 macrophages are very well suited for co-culture systems where you want to model tumor-myeloid or macrophage-T cell interactions. Because they exhibit a strong pro-inflammatory profile, they can be used to investigate how activated macrophages shape tumor cell behavior, impact T cell activation, or modulate cytokine networks in vitro. While we do not provide a fixed "one-size-fits-all" co-culture protocol, we can offer practical recommendations regarding seeding densities, timing of macrophage activation, and media formulations so that you can fine-tune the model to your study objectives.

We recommend a rapid thaw at 37°C until only a small ice crystal remains, followed by immediate transfer to a sterile tube containing pre-warmed culture medium. Slowly dilute the cell suspension to reduce osmotic shock, then centrifuge gently to remove cryoprotectant. After resuspension in fresh macrophage medium and seeding on tissue-culture-treated plastic, cells are usually allowed to recover overnight before stimulation or functional assays.