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Human Macrophages, Alveolar (MTS-0922-JF10)

Datasheet

Overview

Description

This product is derived from healthy human lungs with donors who fully agree. It is negative in the tests of HBV, HCV, HIV-1, mycoplasma, bacteria, fungi, and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Human

Product Type

Human Macrophages

Specification

Cell Source

Alveolar

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for HBV, HCV, HIV-1, mycoplasma, bacteria, fungi, and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the purity of this cell population? How do you confirm they are alveolar macrophages?

These cells are highly purified. We confirm their identity as macrophages by testing for positive expression of the pan-macrophage marker CD11b via flow cytometry. Alveolar macrophages are also often characterized by high autofluorescence and expression of markers like CD206, which you can verify in your own assays.

What are the most common applications for these cells?

Being the primary immune cell in the lung, they are ideal for any in vitro research related to pulmonary inflammation, respiratory diseases (like asthma, COPD, or fibrosis), lung infections (viral or bacterial), and inhalation toxicology. They are perfect for phagocytosis assays, cytokine release profiling, and gene expression studies in response to lung-specific stimuli.

How many cells are in one vial, and what is the expected viability after thawing?

Each vial contains a guaranteed cell count (please refer to the lot-specific datasheet for the exact number, as it can vary). We guarantee high viability upon thawing, provided you follow our handling protocol exactly. This includes immediately storing the vial in liquid nitrogen upon receipt and using the recommended thawing procedure and culture medium.

We were thrilled to find these
These are the best primary alveolar macrophages our lab has ever worked with. We study influenza infection, and the response from these cells was robust and much more physiologically relevant than cell lines. Viability was>95% post-thaw. Worth every penny.
They adhered well and were ready for experiments within 24 hours
We used these for a study on bacterial pneumonia. The cells were highly active and produced a strong pro-inflammatory response upon challenge with S. pneumoniae. The QC data provided gave us great confidence in our results.
Will definitely be ordering again
The donor screening is essential for our lab's safety standards. They performed well in our fibrosis co-culture model. Make sure to follow the handling instructions precisely, as they are sensitive primary cells.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.