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BALB/C Mouse Macrophages, Peritoneal Cavity, 6 x 10^6 (MTS-0922-JF28)

Datasheet

Overview

Description

This product is isolated from the peritoneal cavity of pathogen-free laboratory BALB/C mouse. 6 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

BALB/C Mouse Macrophages

Specification

Cell Source

Peritoneal cavity

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

6 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the functional state of these "elicited" macrophages?

These cells are in a pre-activated, pro-inflammatory state. They are not naïve M0 cells. They are known for their high phagocytic capacity and are primed for a robust inflammatory response.

What is the BALB/c strain used for?

The BALB/c strain has a Th2-biased immune system. These elicited macrophages are therefore a common model for studying macrophage function in the context of parasitic infections (like Leishmania) or allergic inflammation.

Are these "resident" or "elicited" peritoneal macrophages?

This product contains 6 x 10^6 cells, which is a high-yield. This indicates they are thioglycollate-elicited macrophages. The mice were injected with thioglycollate to recruit a large population of activated, inflammatory macrophages to the peritoneal cavity.

We got a very strong inflammatory signal from them
We use these for our in vitro Leishmania infection model, and they work perfectly every time. This saves us the 4-day thioglycollate injection and harvest protocol. A huge time-saver.
Will be using these regularly
Very 'angry' macrophages, just as you'd expect from a thio-prep. High viability. Be aware they are not M0 cells. They are already activated. This was perfect for our needs.
The 6M count is great
Clean, viable, and a large cell count. We use these for studying the resolution of inflammation. The BALB/c background is critical. We're very happy with this product.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.