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Diabetic Mouse Macrophages, Bone Marrow (MTS-0922-JF26)

Q: Why should I use these diabetic macrophages for my research?

These cells are an invaluable in vitro model for studying the cellular mechanisms of diabetic complications. They exhibit "metabolic memory" and have a pre-existing pro-inflammatory and metabolically stressed phenotype, which is ideal for studying impaired wound healing, diabetic neuropathy, or cardiovascular disease.

Datasheet

Overview

Description

This product is isolated from the bone marrow of pathogen-free laboratory mouse. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Why should I use these diabetic macrophages for my research?

These cells are an invaluable in vitro model for studying the cellular mechanisms of diabetic complications. They exhibit "metabolic memory" and have a pre-existing pro-inflammatory and metabolically stressed phenotype, which is ideal for studying impaired wound healing, diabetic neuropathy, or cardiovascular disease.

Are these cells functionally different from healthy BMDMs?

Absolutely. You should expect these macrophages to have a distinct phenotype. They often show a pre-activated M1-like state, impaired phagocytosis, altered lipid metabolism, and a different response to stimuli like LPS compared to cells from a healthy, lean control mouse.

What is the recommended control for these cells?

The ideal control would be our standard Bone Marrow Macrophages from the same genetic background (e.g., C57BL/6, our product MTS-0922-JF41). This allows you to directly attribute any functional differences to the diabetic state.

The gene expression profile was exactly what we'd expect from a diabetic macrophage
High viability and no contamination. A great tool for anyone in the diabetes or metabolism field. Saved us a ton of animal work and protocol optimization.
Much easier than inducing diabetes in-house
Our lab studies metabolic memory, and these cells are perfect. Even after several days in culture, they retained their "diabetic" phenotype. We are very happy with this purchase.
The cells clearly show the pro-inflammatory phenotype we've been trying to replicate
Very good cells. They are functionally very different from our healthy B6 controls. They showed impaired M2 polarization, which was the hypothesis of our study. A bit pricey, but worth it.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.