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CD1 Mouse Macrophages, Spleen, 1-10 x 10^6 (MTS-0922-JF75)

Datasheet

Overview

Description

This product is isolated from the spleen of pathogen-free laboratory CD1 mouse. 1-10 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

CD1 Mouse Macrophages

Specification

Cell Source

Spleen

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 x 10^6 cells, 2 x 10^6 cells, 5 x 10^6 cells, 10 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Are these spleen-derived macrophages suitable for functional assays such as phagocytosis and cytokine release, or are they mainly for phenotypic characterization?

The CD1 spleen macrophages are designed to be fully functional and are not limited to phenotypic characterization. This makes them highly suitable for downstream applications including innate immune signaling studies, drug screening for anti-inflammatory compounds, and co-culture with lymphocytes for evaluating antigen presentation and cross-talk between innate and adaptive immune cells.

Do these CD1 spleen macrophages maintain their phenotype over several days in culture?

When cultured under recommended conditions, these cells usually maintain a stable macrophage phenotype for several days. You will see typical adherence, spreading, and a consistent expression of surface markers during this period. However, as primary cells, they are not intended for long-term passaging like cell lines.

Can I integrate these macrophages into an antigen-presentation setup with T cells, and do you provide any guidance on co-culture conditions?

Yes, these CD1 mouse spleen macrophages are well suited for antigen-presentation and co-culture experiments with T cells. They express MHC-II and co-stimulatory molecules required for effective interaction with T lymphocytes.

We used the CD1 spleen macrophages for a series of innate immune activation studies
Viability after thawing was high, adherence was quick, and the response to LPS was strong and reproducible, which gave us a lot of confidence in the data. Compared with isolating macrophages in-house, this product saved us time and removed a lot of variability from our workflow.
I was pleasantly surprised by how well these primary macrophages recovered after arrival
They attached nicely to culture plates within a few hours and showed classical macrophage morphology. We used them for phagocytosis assays and cytokine profiling, and both sets of experiments produced clean, high-quality data.
These cells helped us standardize our experimental pipeline
Previously, student-to-student variability in isolation and culture affected our macrophage-based experiments. By switching to this off-the-shelf product, we significantly reduced the time spent on animal tissue processing and improved consistency in our readouts.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.