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B129 Mouse Macrophages (Thioglycollate-elicited), Peritoneal Cavity, (MTS-0922-JF19)

Datasheet

Overview

Description

This product is isolated from the peritoneal cavity of pathogen-free laboratory B129 mouse. It is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

B129 Mouse Macrophages

Specification

Cell Source

Peritoneal cavity

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the source and strain of these cells?

These are thioglycollate-elicited peritoneal macrophages isolated from a 129 mouse strain. They are in a pre-activated, inflammatory state due to the thioglycollate injection.

Why would I need the 129 mouse strain?

The 129 strain is the background for many common embryonic stem cell lines used to create knockout mice. If your in vivo studies use a 129-background mouse, these macrophages are the correct genetic control for your in vitro validation experiments.

What is the benefit of "thioglycollate-elicited"?

Elicitation with thioglycollate recruits a very large number of highly phagocytic, inflammatory macrophages. This product gives you a large cell count (6 million) of activated cells, which is ideal for functional assays like phagocytosis, ROS bursts, or inflammasome activation, without needing to stimulate them in vitro.

They were already activated and ready for our functional assay
The 129 strain is a specific requirement, so I'm glad Creative Biolabs offers it. They performed well in our phagocytosis assay. No issues.
129 strain, thioglycollate-elicited, 6 million cells
These cells are potent. They arrived perfectly frozen and thawed with>90% viability. They were already activated and ready for our functional assay. A great time-saver.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.