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C57BL/6 Mouse Macrophages, Lung, 0.5 x 10^6 (MTS-0922-JF14)

Datasheet

Overview

Description

This product is isolated from the lung of pathogen-free laboratory C57BL/6 mouse. 0.5 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

C57BL/6 Mouse Macrophages

Specification

Cell Source

Lung

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

0.5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Are these M0, M1, or M2 macrophages?

As these are resident lung macrophages isolated from a naïve, healthy mouse, they can be considered a resting/M0-like population. They are an ideal starting point for in vitro polarization or stimulation assays.

Can these be used for in vitro infection models?

Absolutely. These primary lung macrophages are an excellent, physiologically relevant model for studying host-pathogen interactions with respiratory viruses (like Influenza or SARS-CoV-2) or bacteria (like M. tuberculosis or S. pneumoniae).

What is the recommended medium for culturing these?

A detailed protocol with our recommended culture medium (typically a DMEM or RPMI-based medium with 10% FBS and specific growth factors like M-CSF) is included with your purchase and on the product datasheet.

The 500k count is perfect for seeding a 24-well plate.
We match these to all our B6 knockout mouse studies. The quality is always top-notch, high viability, and no contamination. Saves us so much prep time.
So much better than using a cell line like RAW 264.7.
They are primary, so they require careful handling, but the protocol from Creative Biolabs is clear and easy to follow. They performed well in our LPS stimulation assay.
The cell count was a little low for our needs
Viability was good. The cell count was a little low for our needs (had to buy 3 vials), but the purity was high. We were able to get clean flow cytometry data from them.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.