Rat Macrophages (with Luciferase expression), Bone Marrow (MTS-0922-JF88)

These macrophages express firefly luciferase, a widely used reporter that requires the substrate D‑luciferin. In vitro assays typically involve adding a luciferin‑containing reagent to cells in multi‑well plates and then measuring emitted light with a luminometer. For in vivo imaging, luciferin is administered systemically, and a dedicated bioluminescent imaging system is used to capture the emitted photons.

We evaluate luciferase‑expressing macrophages against non‑labeled controls in key functional assays, including phagocytosis and cytokine secretion. Under standard culture conditions, no significant differences are observed in viability, morphology, or basic immune responses.

Key considerations include optimizing the number of cells transplanted, choosing the appropriate route of administration, and standardizing luciferin dosing and timing before imaging. Tissue depth and pigmentation can influence signal intensity, so consistent imaging settings and positioning are crucial for meaningful comparisons. We provide general best‑practice guidelines, but the final parameters should be fine‑tuned to fit your specific model and research questions.