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BALB/C Mouse Macrophages, Resident peritoneal Cavity, 0.5 x 10^6 (MTS-0922-JF29)

Datasheet

Overview

Description

This product is isolated from the resident peritoneal cavity of pathogen-free laboratory BALB/C mouse. 0.5 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

BALB/C Mouse Macrophages

Specification

Cell Source

Resident peritoneal cavity

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

0.5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Why is the cell count (0.5 x 10^6) so much lower?

The resident macrophage population in a naïve mouse is much smaller than the population recruited during inflammation. A yield of 0.5 x 10^6 (500,000) cells is a typical and high-quality harvest for resident peritoneal macrophages.

What is the recommended application for these cells?

These are ideal for studying the function of steady-state tissue-resident macrophages, as a starting population for polarization assays, or as a direct control for the "elicited" macrophage population (JF28).

Can these cells be polarized to M1 or M2?

Yes. Since these are naïve M0-like cells, they are an excellent starting population for in vitro polarization studies. You can use them to compare M1/M2 polarization in a Th2-biased (BALB/c) genetic background.

Resident peritoneal macrophages from a BALB/c mouse
A fantastic source for naïve BALB/c macrophages. They adhered well and were ready for stimulation. We got a very clean M2 polarization with IL-4.
These were a perfect M0 control for our polarization assay
We used these as our baseline control to compare against the thioglycollate-elicited macrophages. The functional and gene expression differences were night and day. A great product for this kind of comparison.
The count is low (500k), so they are best for 24-well or 48-well plates
A fantastic source for naïve BALB/c macrophages. They adhered well and were ready for stimulation. We got a very clean M2 polarization with IL-4.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.