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Human M2 Macrophages, Peripheral Blood, 10 x 10^6 (MTS-0922-JF7)

Datasheet

Overview

Description

This product is isolated from human peripheral blood. Donors are screened for hepatitis B, hepatitis C, HIV-1 and HIV-2. 10 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Human

Product Type

Human M2 Macrophages

Specification

Cell Source

Peripheral blood

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

10 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the main advantage of this product (MTS-0922-JF7)?

This product provides a larger quantity of cells, 10 x 10^6 (10 million) per vial, compared to our 2 million cell-count vials. This is ideal for researchers who need to run larger-scale experiments, plate multiple high-density plates, or require more cells for downstream applications like Western blotting or RNA extraction.

What is the recommended culture medium for these cells post-thaw?

We strongly recommend using the specific macrophage culture medium detailed in the protocol provided with your shipment. This medium is optimized to maintain the viability and M2 phenotype of the cells.

What is the cell source?

These are Human M2 Macrophages derived from peripheral blood monocytes (PBMCs) of a healthy adult donor.

Love the 10 million cell vial!
It's so much more convenient for our workflow. We can run a full 96-well plate assay and still have cells left for flow cytometry. The quality is fantastic, as always.
They worked perfectly. Great quantity.
We use a lot of M2 macrophages, and this 10 million cell vial is a lifesaver. The quality is consistent, and they perform well in functional assays. Thawed great, with>90% viability.
The large volume is very economical for a high-throughput lab
They were perfect for our wound healing assay, promoting fibroblast migration as expected. High viability and a great cell count for the price. This is our new go-to bulk M2 product.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.