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M1 Macrophage Generation Medium (MTS-1223-HM2)

Datasheet

Overview

Description

Creative Biolabs offers serum-free and xeno-free medium for generation of M1 macrophages from fresh monocytes. This product contains GM-CSF and allows for the generation of M1-polarized macrophages.

Applications

Cell Culture

Product Type

Medium

Specification

Component

Basal Medium M1; SupplementMix M1; CytokineMix M1: GM-CSF

Size

250 mL

Handling Notes

Do not freeze the Basal Medium. Do not freeze the complete medium.

Storage

Basal Medium at 4-8°C in the dark; SupplementMix at -20°C immediately after arrival. Cytokine Mix at -20°C for long-term storage or at 4-8°C for up to 2 weeks.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Does the medium come as a complete solution, or do I need to add extra cytokines such as IFN-γ or LPS?

The M1 Macrophage Generation Medium is supplied as a ready-to-use formulation that includes the necessary components to drive M1 polarization. In many protocols, no additional cytokines are required to establish a strong M1 profile. However, some users may choose to add specific stimuli for customized or intensified polarization, especially when modeling particular disease-relevant conditions.

Is this medium compatible with monocyte-derived macrophages from different donors or species?

Yes. The formulation has been successfully used to induce M1 phenotypes in human monocyte-derived macrophages as well as in certain rodent models. While the exact expression levels of markers and cytokines may differ between donors or species, the overall shift toward a pro-inflammatory state is consistent. We recommend minor empirical adjustments in culture time or seeding density when working with new sources.

Are there any considerations for using this medium in high-throughput or imaging-based assays?

The medium is compatible with standard multi-well culture formats and imaging workflows. To maximize reproducibility, we recommend optimizing seeding density, ensuring uniform distribution of cells, and providing adequate recovery time before polarization. For imaging, it can be useful to transition to imaging-compatible medium after phenotype induction, depending on your microscope and fluorophore requirements.

This M1 medium has made our polarization workflow much more straightforward
We can start from M0 macrophages and obtain a reproducible M1 phenotype within two days, with robust cytokine responses and clear marker expression. It has reduced our dependence on multiple separate cytokine reagents.
It integrates very well into our 96- and 384-well platforms
Our lab uses this medium to generate M1 macrophages for small-molecule screening. The phenotype is strong and consistent, and assay variability has decreased compared with our previous in-house protocol.
It has become part of our standard toolbox for immune assay development
Overall, this M1 generation medium is an excellent tool for inflammatory macrophage research. The documentation is clear, and the product behaves exactly as advertised.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.