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B6/129 Mouse Macrophages, Bone Marrow (MTS-0922-JF36)

Datasheet

Overview

Description

This product is isolated from the bone marrow of pathogen-free laboratory B6/129 mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

B6/129 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the advantage of using an immortalized line?

Immortalized cells provide a consistent, renewable resource for your experiments. This eliminates the lot-to-lot variability associated with primary cells and removes the need for repeated animal harvesting, making your long-term studies more reproducible.

What is the background strain B6/129 useful for?

The B6/129 mixed background is very common in transgenic and knockout mouse models. These cells are an excellent control line to use alongside macrophages derived from B6/129-background knockout mice.

A fantastic immortalized line
We were tired of the variability in our primary BMDMs. These cells grow robustly and respond consistently to LPS stimulation. A real workhorse for our lab.
The immortalization is very effective
We needed a control line for our B6/129-background KO model, and these were perfect. They behave very similarly to primary BMDMs in our functional assays but are so much easier to handle.
We've already re-ordered twice
The CD11b expression was high. My only note is to make sure you use their recommended medium; they didn't do as well in our standard lab-made macrophage medium.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.