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Human M1 Macrophages, peripheral blood-derived (MTS-1223-HM7)

Datasheet

Overview

Description

Creative Biolabs offers frozen, peripheral blood-derived M1 Macrophages.

Species

Human

Product Type

Cell

Specification

Cell Source

Peripheral Blood

Size

1,500,000 cells

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Are these cells compatible with gene editing or viral transduction workflows before or after polarization?

Yes, many groups work with these cells as part of genetic manipulation workflows. In general, gene editing or viral transduction is most efficient at the monocyte or early macrophage stage prior to full M1 polarization, but some protocols can still be adapted post-polarization. Because transduction conditions vary widely, we recommend that you perform a pilot test to optimize MOI, exposure time, and any selection steps.

Can these peripheral blood-derived M1 macrophages be used to model chronic inflammatory states, or are they best suited for acute activation studies?

They are very effective for acute activation studies, where you want a robust pro-inflammatory response following a defined trigger.

Do you provide technical support if I encounter issues with recovery or assay performance?

If you encounter any difficulties-such as lower-than-expected recovery, morphological changes, or weaker responsiveness to stimuli-our scientific support team is available to help troubleshoot. We can review your workflow, suggest adjustments, and, if necessary, help determine whether a technical replacement may be appropriate.

We needed primary human M1 macrophages that actually behaved like cells from peripheral blood rather than immortalized surrogates
These cells responded very strongly in our LPS/IFN-γ challenge model, with clean and reproducible cytokine profiles across replicates. The donor information and QC report made it easy to include them in regulatory documentation for a sponsored study. Overall, they gave us the confidence to run a fairly complex inflammatory panel without constantly worrying about baseline variability.
Considering the complexity of primary human cells, the overall performance and reliability were very good
Our project involved evaluating small molecules for their ability to dampen innate immune activation. Using these peripheral blood-derived M1 macrophages, we could clearly differentiate between weak, moderate, and strong inhibitors based on TNF-α and IL-6 levels. The cells maintained a stable phenotype for several days, allowing us to repeat dosing and sampling across a short time course.
After a positive experience with a small initial order, we scaled up to support a larger screening campaign without any major issues
As an early-stage biotech company, we don't have the resources to isolate and polarize macrophages in-house. This product effectively outsourced that part of the workflow for us. We were able to focus on our assay development and data analysis, knowing that the M1 cells came from a consistent and professionally controlled process.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.