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Human M2 Macrophages, Peripheral Blood (Age: 47), 2 x 10^6 (MTS-0922-JF4)

Datasheet

Overview

Description

This product is isolated from human peripheral blood. Donors are screened for hepatitis B, hepatitis C, HIV-1 and HIV-2. 2 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Human

Product Type

Human M2 Macrophages

Specification

Cell Source

Peripheral blood

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

2 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Are these cells already polarized to the M2 phenotype?

Yes, this product consists of human macrophages derived from peripheral blood monocytes that have been differentiated and polarized in vitro to the M2 (alternatively activated) phenotype. They are ready to use for your experiments.

How many cells are in each vial?

This specific product (MTS-0922-JF4) contains 2 x 10^6 (2 million) viable cells per vial, delivered frozen in a cryopreservation medium.

What is the significance of the donor age (47)?

We provide donor age and other demographic information (when available) to allow for more controlled and specific experimental designs, particularly for research on age-related diseases, immunology, or drug metabolism. This lot is specifically from a 47-year-old donor.

The 2 million cell count was a bit low for our needs, but the quality was high
These M2 macrophages were perfect for our tissue repair model. We confirmed high CD206 expression upon arrival. Having the donor age was a huge plus for our study on aging. Great viability.
Creative Biolabs is our go-to for polarized macrophages
These M2s from the 47-year-old donor were exactly what we needed. They performed beautifully in our functional assays. No contamination, high purity.
The cells are definitely M2, showing the typical elongated morphology and marker expression
Used this batch for a study on macrophage-fibroblast interactions. The cells were highly viable and maintained their M2 phenotype in our culture conditions for several days. The safety screening data was essential for our lab's work.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.