Langerhans cells, Skin and Oral Mucosa (MT-0224-HM20)

Absolutely. The cells integrate well into reconstructed epidermal models and can be added during or after tissue formation, depending on the experimental design. Within these 3D systems, they help recapitulate tissue-resident immune surveillance and enable more physiologically relevant responses to stimuli. Our team can share practical tips on seeding strategies and culture conditions for maintaining viability and function in complex skin equivalents.

We employ an optimized cryopreservation protocol and validate post-thaw function by measuring antigen uptake, processing, and T-cell activation in internal tests. Viability and key surface markers are also assessed. Only batches that maintain adequate antigen-presenting capacity under our standard thawing conditions are released, helping customers obtain reliable results from assays focused on adaptive immune activation.

Yes. On request, we can pre-activate Langerhans cells with defined stimuli or pre-load them with model or custom antigens, followed by basic functional validation to confirm viability and activation status. This option is useful for clients who want to reduce hands-on time or ensure standardized activation conditions across multiple experiments, particularly in high-throughput or regulatory-oriented studies.