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Canine Macrophages, Bone Marrow, 6 x 10^6 (MTS-0922-JF1)

Datasheet

Overview

Description

This product is isolated from the bone marrow of pathogen-free laboratory canine. 6 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Canine

Product Type

Canine Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

6 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the expected viability of these cells upon thawing?

We guarantee high viability upon arrival, provided the cells are handled immediately and correctly according to our provided protocol. This includes transferring them directly to liquid nitrogen upon receipt and using the recommended thawing procedure.

What culture medium do you recommend for these bone marrow-derived macrophages?

A detailed protocol with our recommended culture medium formulation is included with your purchase and is available on the product datasheet. Using the recommended medium is crucial for optimal cell performance and viability.

Can these cells be used for in vivo studies?

This product is sold for "Research Use Only" and is primarily intended for in vitro applications such as morphological studies, gene expression analysis, and physiological assays. They are not suitable for human or animal

Excellent quality cells
We used these for a phagocytosis assay and the results were highly consistent. Viability was>90% post-thaw. Will be purchasing again for our canine immunology studies.
Good cell count and purity
The CD11b expression was strong. We had a slight learning curve with the recommended medium, but the technical support team was very helpful. Overall, a reliable source for canine primary cells.
The QC data provided peace of mind
These macrophages responded well to LPS stimulation in our gene expression study. It's difficult to find high-quality canine primary cells, and these met all our expectations.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.