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C57BL/6 Mouse Macrophages (with BAX knockout), Bone Marrow (MTS-0922-JF38)

Datasheet

Overview

Description

With BAX knockout, this product is isolated from the bone marrow of pathogen-free laboratory C57BL/6 mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

C57BL/6 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the significance of the BAX knockout in these cells?

BAX is a pro-apoptotic protein, a key member of the Bcl-2 family. Macrophages lacking BAX are resistant to certain apoptotic pathways. These cells are an invaluable tool for studying apoptosis, cell survival, and the role of BAX in macrophage function, such as in response to pathogens or stress signals.

Has the BAX knockout been validated?

Yes, the genetic modification (BAX knockout) is a feature of the source mice. The cells are then quality controlled for being free of microbial contaminants and for the expression of the CD11b macrophage marker.

Can I use these alongside a wild-type control?

Absolutely. We recommend using our standard C57BL/6 Bone Marrow Macrophages (Cat# MTS-0922-JF40) as the ideal wild-type control, as they are from the same background and immortalized using the same method.

This cell line was exactly what we needed for our apoptosis study
We treated them with staurosporine alongside the C57BL/6 wild-type line (JF40) and saw a significant, expected resistance to cell death. The data is publishing-quality.
Great control cells for apoptosis research
Saved us the immense trouble of crossing BAX-KO mice and isolating primary cells. The cells are healthy, grow well, and are confirmed BAX-deficient.
They arrived in great condition. Highly specialized and effective tool
Excellent cell line. Confirmed CD11b+ and F4/80+ in our lab. They are, as advertised, highly resistant to intrinsic apoptosis. We are very happy with this purchase.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.