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129 Mouse Macrophages (with IFN-α/β receptor knockout), Bone Marrow (MTS-0922-JF44)

Datasheet

Overview

Description

With interferon-α/β receptor knockout, this product is isolated from the bone marrow of pathogen-free laboratory 129 mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

129 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the genetic background of these macrophages?

These macrophages are derived from the 129 mouse strain. This is an important detail as the 129 strain has a distinct genetic background from other common strains like C57BL/6, and it's crucial for researchers who are conducting comparative studies or working within a 129-strain-specific model.

Can you confirm which interferon receptor is knocked out in this cell line?

Yes, this cell line features a knockout of the Type I interferon-α/β receptor (IFNAR). This makes these cells unresponsive to Type I interferons (IFN-α and IFN-β) and an invaluable tool for studying viral infections or inflammatory pathways where Type I IFN signaling is a key variable.

Are these cells suitable for studying Type II interferon (IFN-gamma) responses?

Absolutely. Since only the Type I (IFN-α/β) receptor is knocked out, the Type II interferon receptor (IFNGR) remains intact and functional. This makes the cell line an ideal negative control system to isolate and study IFN-gamma-specific signaling pathways without confounding activation from endogenous or experimental Type I IFNs.

Excellent research tool
High viability, clean culture, and the knockout is solid. We used these alongside the wild-type 129 macrophages from CB, and the data is incredibly clean. We can clearly see the IFN-alpha/beta-dependent gene expression.
A stable 129-background IFNAR knockout macrophage line
The cells are growing well and are definitely from a 129 background. They performed well in our viral infection model. It would be great if the datasheet came with a sample growth curve, but otherwise, a solid product.
This is a crucial control for our innate immunity experiments
The cells are easy to handle for an immortalized line. We are using them to study IFN-gamma pathways, and it's a relief to not have to worry about cross-talk from any potential Type I IFN contamination. Viability was>90% on arrival.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.