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B129 Mouse Macrophages, Peritoneal Cavity, 0.5 x 10^6 (MTS-0922-JF21)

Datasheet

Overview

Description

This product is isolated from the peritoneal cavity of pathogen-free laboratory B129 mouse. 0.5 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

B129 Mouse Macrophages

Specification

Cell Source

Peritoneal cavity

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

0.5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the difference between this product (JF21) and your other 129-strain product (JF19)?

This product (MTS-0922-JF21) consists of resident peritoneal macrophages from a naïve 129 mouse, and it contains 0.5 x 10^6 cells. Product MTS-0922-JF19 consists of thioglycollate-elicited (activated) macrophages and contains a much higher cell count (6 x 10^6). You should choose this product if you need a naïve, M0-like population.

What is the cell count?

This vial contains 0.5 x 10^6 (500,000) viable cells. This quantity is suitable for smaller-scale experiments, such as 24-well or 48-well plate assays, or for flow cytometry.

Can these cells be polarized to M1 or M2?

Yes. As naïve, resident (M0) macrophages, they are an excellent starting population for in vitro polarization experiments. They will respond readily to standard cytokine cocktails (e.g., LPS/IFN-γ for M1, IL-4/IL-13 for M2).

Good for pilot studies
Good quality, but be aware of the 500k cell count. It's not a lot of cells. We had to buy several vials for our experiment. The cells themselves were great, though.
Exactly as advertised
We successfully polarized them to M1 and M2. The protocol was helpful. Will likely buy the larger "thio" elicited vial next time if we need more cells, but these were great for establishing the model.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.