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Rat Macrophages & Microglia Cells Medium (MTS-1223-HM11)

Datasheet

Overview

Description

Creative Biolabs offers freezing medium with higher FBS concentration, more suitable for macrophages & microglia Cells.

Applications

Cell Culture

Product Type

Medium

Specification

Size

50 mL

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What types of cells is the Rat Macrophages / Microglia Cells Medium specifically optimized for, and can it be used for both primary cells and cell lines?

This medium is tailored to support the growth and maintenance of rat-derived macrophages and microglia, with particular attention to their unique metabolic and functional requirements. It has been used successfully with primary microglia isolated from rodent CNS tissue as well as with bone marrow-derived macrophages and certain macrophage-like cell lines. The formulation balances nutrients, buffering capacity, and growth-supporting factors in a way that encourages cell survival and preserves relevant phenotypic features, including ramified morphology in microglia. While primarily optimized for primary cells, many users also find it beneficial as a base for culturing related cell lines, often with minor adjustments or additional supplements.

Is the medium serum-free or does it require serum supplementation, and can I adapt it to my preferred serum source?

The exact format is described in the product datasheet, but in general, the medium can be used either as a complete formulation or with specified serum supplementation as indicated. Many users appreciate the flexibility to use their preferred serum source, especially when working with long-standing experimental protocols or institutional standards.

Can this medium support functional assays such as cytokine release, phagocytosis, or calcium imaging in microglia and macrophages?

Yes. The medium is compatible with a wide range of functional assays commonly used in microglia and macrophage research. Users regularly employ it in cytokine-release studies following stimulatory treatments, phagocytosis assays using labeled particles, and live-cell calcium imaging to monitor signaling events.

We tested this medium in a project that combines microglia with neurons in co-culture
The medium balanced the needs of both cell types remarkably well; microglia stayed healthy and responsive, while neurons remained viable and displayed normal morphology and activity. This has allowed us to study microglia-neuron crosstalk in a more physiologically relevant setup, which is crucial for our disease models.
Using this medium, we observed clear differences between resting and activated microglia that were easier to quantify
In our high-content imaging assays, microglial morphology is a critical readout. Using this medium, we observed clear differences between resting and activated microglia that were easier to quantify. The reduced background activation at baseline meant that any changes we saw were more clearly attributable to our experimental treatments, which strengthened the conclusions of our study.
We've used it successfully in both short- and medium-term experiments and have found it consistently reliable
Working with primary rodent cells can be challenging, especially when multiple users share the culture facility. This medium has been forgiving enough to tolerate minor variations in technique while still supporting robust cell health.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.