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C57BL/6 Mouse Macrophages (with iNOS knockout), Bone Marrow (MTS-0922-JF48)

Datasheet

Overview

Description

With inducible nitric oxide synthases (iNOS) knockout, this product is isolated from the bone marrow of pathogen-free laboratory C57BL/6 mice after immortalization with a recombinant retrovirus, which contains the v-raf and myc oncogenes. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

C57BL/6 Mouse Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What are the primary applications for these iNOS KO macrophages?

These cells are essential for any research aiming to separate the effects of nitric oxide from other M1 macrophage functions. They are widely used in studies of host-pathogen interactions (to see if NO is required for pathogen killing), inflammation, and signal transduction.

Do these cells still polarize to an "M1" phenotype in other ways?

Yes. The Nos2 knockout is very specific. When stimulated with IFN-gamma and LPS, these cells will still upregulate other key M1 markers, such as pro-inflammatory cytokines (e.g., TNF-α, IL-6, IL-1β) and co-stimulatory molecules. This makes them a precise tool for studying NO-independent M1 functions.

What gene is knocked out in this cell line?

This cell line has a knockout of the Nos2 gene, which codes for inducible nitric oxide synthase (iNOS). iNOS is a key enzyme produced by M1-polarized macrophages that generates large amounts of nitric oxide (NO) as a defense mechanism against pathogens and in inflammatory responses.

The knockout is 100% functional. Excellent
These iNOS KO cells are fantastic. They grow robustly, are free of contamination, and come from the C57BL/6 background we needed. It's so much more convenient than harvesting BMDMs from our Nos2 -/- colony.
Good, reliable cell line
We confirmed they still produce plenty of TNF-alpha, so the rest of the M1 pathway is intact. This allows us to cleanly study the downstream signaling of NO. Viability on arrival was great.
A crucial control for our inflammation models
Shipping was fast. The cells perform exactly as advertised. My only minor critique is that the datasheet could be more detailed, but the recommended medium works perfectly.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.